ISSN 2413-7642
А.V. Pylypchenko, president of the Institute of organic agriculture, LLC
M.B. Piskovyi, candidate of agricultural sciences, deputy director of the Institute of organic agriculture, LLC
M.M. Orlov, candidate of agricultural sciences, laboratory manager of the Institute of organic agriculture, LLC
А.V. Cvirinko, student, assistant of the laboratory manager of the Institute of organic agriculture, LLC
Poltava, Ukraine
Improvement of the breeding process in hemp growing to saturate the varieties with useful cannabinoids
Today the group of companies “Arnika”, Hlobyne town, Poltava region, is one of the largest companies in Ukraine and in the world that grows agricultural crops using organic farming technologies.
It is clear that in the transition from classical technologies to organic production there were a number of problems to solve which had to use both existing experience and new research. One of the branches of agriculture in Ukraine, which best meets the requirements of organic farming, is hemp growing. The biological properties of the crop play an important role in this matter, namely that they require almost no chemical protection against pests and diseases.
The scope of raw materials from hemp is significantly expanding. Besides the traditional use in light, construction, pulp and paper, automotive and aircraft, chemical industries, hemp products began to be used for the manufacture of medicines, perfumes, cosmetics, high-quality oil, additives to many foods and others.
It is the latest uses of hemp that require the use of environmentally friendly products that would be of high quality, greater demand and cost.
The creation of varieties and hybrids of plants that meet the needs of the market is devoted to the research conducted during 2014-2020 and still currently continues.
In this article, we have tried to show our research, achievements and conclusions.
Initially, the plants with a reduced content of THC (0.01-0.001%) and an increased content of CBD (0.50-1.50%) and CBG (0.02-0.70%) in the variety Zolotoniski 15 were selected, and later the breeding work was continued with the varieties Globa, Lara, Sula and a number of future varieties that will be submitted for the state variety testing.
In the first three years, the targeted selection in the direction of increasing the content of CBG in cannabis plants proved to be quite effective. But life sets new challenges and our search continues.
Key words: cannabinoids, tetrahydrocannabinol (THC), cannabinol (СBN), cannabidiol (CBD), cannabigerol (CBG), cannabichromene (CBC), enzymes, precursors, breeding, technical hemp, variability, correlation.
REFERENCES
- Nykolskyi, B.N. (Eds.). (1971). Spravochnyk khymyka. T. 2. Osnovnі svoistva neorhanycheskykh y orhanycheskykh soedynenyi (pp. 708-709). Lenynhard: Khymyia
- Lazurievskyi, G.V. & Nikolaeva, L.А. (1972). Kannabinoidy (narkoticheskie veshestva konopli). Kishynev: Shtinica.
- Fulton C.C. (1942). A chemical development of Cannabis sativa L. Industr. and Chem.Analyt. Ed.,14, 404-406.
- Fettermann R.S., & Turner J.C. (1972). Constituents of Cannabis sativa L. I.J/1 propel. Homologue of Cannabinoids from an Indian variantant. J.Pharm.Sci., 61/9a, 1476–1478.
- Lanyon V.S., Turner J.C., & Vahlberrg P.G. (1981). Quantitative analysis of cannabinoids in the secretory product from capitates-stalked grands of Cannabis sativa L.(Cannabaceae) Bot. Gaz., 142, 3a, 316–319.
- Dajani E.Z., Larsen K.R., & Taylor J.et al. (1999). Sativex. J. Pharm. Exp. Ther., 291, 31–38.
- Morgan D.R. (1997). Therapeutic uses of Cannabis. Harwood Academic Publishers. Amsterdam.
- Ferenczy L., Gracza L., & Jakobey I. (1958). An antibacterial prepartum from hemp (Cannabis sativaL.) Naturw issens chaften, 45, 188.
- Leizer C., Ribnicky D., & Poulev A. (2000). The composition of hemp seed oil and its potential as an important source of nutrition. Journal of Nutraceuticals, Functional & Medical Foods., 2 (4), 35–53.
- Formukong E.A., Evans A.T., & Evans F.J. (1988). Analgesic and anti-inflammatory activity of constituents of Cannabis sativa L. Inflammation, 12 (4), 361–371.
- Zuardi A.W., Crippa J.A.S., & Hallak J.E.C. [et al]. (2006). Cannabidiol, a Cannabis sativa constituent, as an antipsychotic drug. Brazilian Journal of Medical and Biological Research, 39, 421–429.
- Campos Alline Cristina, Moreira Fabricio Araújo, Gomes Felipe Villela, Aparecida Del Bel Elaine, Guimarães Francisco Silveira, & Philos Trans R Soc Lond B. (2012). Multiple mechanisms involved in the large-spectrum therapeutic potential of cannabidiol in psychiatric disorders. Biol Sci., 5, 367(1607), 3364–3378.
- Leweke F.M., Piomelli D., Pahlisch F., Muhl D., Gerth C.W., Hoyer C., Klosterkötter J., Hellmich M., & Koethe D. (2012). Cannabidiol enhances anandamide signaling and alleviates psychotic symptoms of schizophrenia. Transl Psychiatry, 2(3): e94. Published online.
- Cannapedia. Retrieved from https://olkpeace.org/cannapedia/
Kannabinoidy/KBG.html - What is Cannabigerol (CBG)? High Times. Retrieved from https://www.hightimes.com/read/grow-hack-what-cannabigerol-cbd.
- Technicheskaya konoplya v Ukraine i drugih stranah. Retrieved from Rezhym dostupu: tru.org.ua/nems/4013
- Hrabovski L. (2017). Vmist kanabiherolu s sortakh konopel zakhidnoievropeisk i ukrainskoi selektsii. Dopovid na mizhnarodnii konferentsii «Cannabis». Varshava.
- Davydiuk P.P., Vartuzov V.V., Posilskyi O.O., Zamoshets O.P., Kakhanovskyi F.M., Stelmakhovych S.I., & Meleshko R.A. (2009). Mizhvidomcha metodyka doslidzhennia narkotychnykh zasobiv z roslyn konopel ta maku snotvornoho: metod. posib. Kyiv.
- Standartna operatsiina protsedura SOP.DSE.19/124/2-5.4-4.02 «Yakisne ta kilkisne doslidzhennia narkotychnykh zasobiv, psykhotropnykh rechovyn yikh analohiv ta prekursoriv metodom hazovoi khromato-mas-spektrometrii» VDMRV Cherkaskoho NDEKTs MVS Ukrainy.
- Shkurdoda S.V., Pasichnyk V.V., Orlov M.M., & Piskovyi M.B. (2015). Selektsiia konopel dlia stvorennia sortiv z pidvyshcheknym vmistom kanabidiolu. Visn. KhNAU, Seriia «Roslynnytstvo, selektsiia, nasinnytstvo, plodoovochivnytstvo i zberihannia». Kharkiv, 2, 210–220.
- Metodika gosudarstvennogo sortoispytaniya selskohozyastvennyh kultur. (1972). Moscow: Kolos, 3, 240 (in Russian).
- Shkurdoda S.V., Pasichnyk V.V., & Korol K.P. (2019). Yakisne ta kilkisne vyznachennia kannabinoidiv u chastynakh roslyn konopel metodom khromato-mas-spektrometrii: metodychni rekomendatsii. Kyiv: DNDEKTs MVS.